Sunday, January 26, 2020
Why Dunkin Donuts Closed In Faisalabad Marketing Essay
Why Dunkin Donuts Closed In Faisalabad Marketing Essay James Beard (1903-1985) The food industry is the complex, global collective of diverse business that together supply much of the food energy consumed by the world population. Processed food sales worldwide are approximately 3.2 trillion US dollars (2004). Only in US, consumers spend approximately US 1 trillion dollars. Nestlà © is the largest food and beverage company in the world. The food and its allied products industry is considered Pakistans largest industry. Trade sources estimate the sectors total value of production is over Rs 46 billion. Fast food is the term given to food that can be prepared and served very quickly. In 1867, Charles Feltman, a German butcher opened the first fast food diner in Brooklyn, New York city. Dunkin Donuts is the worlds largest coffee and backed goods chain, serving more than 3 million customers per day. It was founded in 1950 by Bill Rosenberg. The first Dunkin Donuts shop was opened in Quincy, Massachusetts, USA. In 1955 first Dunkin Donuts franchise was sold and ten years later (i.e. 1965) the first international branch was opened in Canada. At the end of 2008, Dunkin Donuts had a total of 8835 franchised restaurants including 2440 international shops in 31 countries. Dunkin Donuts has sales of over 4 million donuts a day and it had sales of 5.5 billion US dollars in the fiscal year 2008. Its international head office is located in Canton, Massachusetts. In 1998, First branch of Dunkin Donuts was opened in Karachi. In January 2004, Dunkin Donuts opened its branch in Faisalabad and it closed down in April 2005. Problem Statement / Research Question Why Dunkin Donuts closed down in Faisalabad Possible Reasons: The Closure of Dunkin Donuts Faisalabad Branch could have been due to one or more following reasons: Inadequate awareness among people of Faisalabad? Failed to develop taste? Difficult Accessibility? Supply Issues? Service Quality? Environment? High Pricing? Methodologies / Study We used to methods in our research process: In-depth telephonic interview Survey through questionnaire In-depth telephonic interview: We obtained the number of the required person from the Dunkin Donuts head office in Lahore. We conducted a telephonic conversation with Mr. Naveed Malik, who was the branch manager of Dunkin Donuts in Faisalabad at the time of closure and currently he is working as the Business Development Manager in Dunkin Donuts. Firstly we inquired him the problem s which contributed in the closure of the branch. He mentioned various reasons to it. According to him they faced supply problems as the kitchen was in Lahore and the stuff reached the branch after 2 and half hours of the daily opening which contributed towards the customer dissatisfaction. He mentioned that they used various tactics to create the awareness among the locals but they were failed in doing so. He also added that Dunkin Donuts did not match the Faisalabads taste culture and they also failed to develop the taste. He also blamed the local city government for their non-supportive behavior towards them as they refused to cut a t urn on the road which made the customers to reach the branch with difficulty. Then we asked him about their competitors in Faisalabad. He replied that there was no competition in Faisalabad as they deal primarily in cafà © and donuts which no other brand offered at that time locally. Then we asked him about their target customers. He told us that the target customers were mainly families Survey through Questionnaire: For the survey we had a sample size of 100 individuals which comprised of our friends, relatives and some strangers. We got the questionnaire (see appendix 1) filled at their homes where we were present in person. The reason behind selecting this target sample was they were easily accessible and were expected to provide their honest opinion as they share personal relationship with us. Our analysis of the questionnaire is as under: Note: For Pie Charts, See Appendix 2 Q 1. What is your Age group? 5 people were of the age less than 10 years, 15 belonged to the age group of 11-15 years, 30 people were aged between 16 and 20, 25 people belonged to the age group of 21-25 and 17 were from the age group of 26-30. Only 8 people were of the age of more than 30 years. Q 2. Do you Like Donuts? 70 people said that they like donuts and 30 people said that they are not fond of donuts. Q 3. Have you ever heard of Dunkin Donuts? 90 people said they had heard of Dunkin Donuts while 10 people had never heard about it. Q 4. Have you ever went to Dunkin Donuts, Faisalabad? Out of the 100 people who answered the questionnaire, 90 had gone to Dunkin Donuts Faisalabad while 10 had not. Q 5. What did you order? 60 people had ordered donuts, 50 had ordered sandwiches, 30 people had offered coffee/tea and 95 people ordered soft drinks. Q 6. How was your overall experience at Dunkin Donuts, Faisalabad? 20 people said that their experience in Dunkin Donuts was excellent, 50 said it was good, 15 said it was fair and 5 said that their experience was bad. Q 7. How will you rate Dunkin Donuts, Faisalabad from scale 1-10 with 10 being the highest (excellent taste)? Mostly people rated Dunkin Donuts between 5 and 8 on a scale of 10. Q 8. Did you go to any of the Dunkin Donuts branches outside Faisalabad? Only 36 people had gone to Dunkin Donuts outside Faisalabad. The other 64 had never been to any Dunkin Donuts branch outside Faisalabad. Q 9. Did you find any difference between the Dunkin Donuts, Faisalabad and the other branches outside Faisalabad which you visited? 6 people said they noticed a lot of difference between Dunkin Donuts branch in Faisalabad and a branch outside Faisalabad. 20 noticed a minor difference and 10 did not find any difference at all. Q 10. What Factor contributed for the difference between Dunkin Donuts, Faisalabad and the other branches? 2 people said that the factor that contributed to the difference between the branches was due to variance in service quality, 10 said it was food quality, 10 said it was environment, 3 said it was cleanliness while 1 person said it was variety of food. Q 11. Can Dunkin Donuts Compete with its competitors? 40 people said yes, 5 people said maybe and 45 people said no. Q 12. Why do you think Dunkin Donuts, Faisalabad closed down permanently? 10 people said the reason for the closure of the branch was bad service, 3 people said unhygienic conditions, 20 people said expensive, 7 people thought it was low food quality, 5 people said environment was not suitable, 5 people said bad location and 40 people said that Dunkin Donuts failed to develop taste of people living in Faisalabad. Limitations Many problems were faced during the preparation of this research project, which were handled amicably. Following were the limitations we have faced during the project In availability of internet Difficulty in finding suitable consultant Load shedding Transportation problem Faced difficulties in printing Conclusion When a customer comes to a restaurant he looks for a mix of services, quality, taste, satisfaction, accessibility and the value of money. If the particular restaurant fails to provide that mix, there are great chances of the restaurant to close down because the customer will become dissatisfied and he will not return to that place again. Same thing happened in the case of Dunkin Donuts Faisalabad, where they failed to provide the mix to their customers. Suggestions: To Enhance supplies To expand their customer base from families to masses. Takes appropriate steps regarding the awareness of the taste among the locals Make the outlet more accessible for the customers Price range should be vast so that it can absorb all income groups Appendix 1 Sample Questionnaire: Name: Contact No: Note: Please Circle the correct answer. You may choose more than one answer Q1) What is your Age group? Less than 10 11-15 16-20 21-25 25-30 30 or more Q2) Do you Like Donuts? Yes No Q3) Have you ever heard of Dunkin Donuts? Yes No If No then you dont need to go further Q4) Have you ever went to Dunkin Donuts, Faisalabad? Yes No If No then skip Q5-6 Q5) What did you order? Donut Sandwich Coffee / Tea Soft Drink Q6) How was your overall experience at Dunkin Donuts, Faisalabad? Excellent Good Fair Bad Q7) How will you rate Dunkin Donuts, Faisalabad from scale 1-10 with 10 being the highest (excellent taste)? _____ / 10 Q8) Did you go to any of the Dunkin Donuts branches outside Faisalabad? Yes No If yes then please specify: ________________ If No, then skip Question 9-10 Q9) Did you find any difference between the Dunkin Donuts, Faisalabad and the other branches outside Faisalabad which you visited? Yes, a lot of difference A minor difference Not at all If you answer is option c then skip Question 10 Q10) What Factor contributed for the difference between Dunkin Donuts, Faisalabad and the other branches? Service Food Quality Cleanliness Environment Variety Q11) Can Dunkin Donuts Compete with its competitors? Yes absolutely Maybe No Q12) Why do you think Dunkin Donuts, Faisalabad closed down permanently? Failed to develop the taste of people living in Faisalabad Bad service Unhygienic Conditions Expensive Low food quality Environment was not suitable Bad selection of location Other: Any suggestion you would like to give regarding Dunkin Donuts? Appendix 2 Pie Charts:
Saturday, January 18, 2020
Caring for individuals with additonal needs
The medical model of disability views disability as a ââ¬Ëproblemââ¬â¢ that belongs to the disabled individual. It is not seen as an issue to concern anyone other than the individual affected. For example, if a wheelchair using student is unable to get into a building because of some steps, the medical model would suggest that this is because of the wheelchair, rather than the steps. However the social model of disability would see the steps as the disabling barrier. This model has the idea that it is society that disables people, through designing everything to meet the needs of the majority of people who are not disabled.There is a realization within the social model that there is a great deal that society can do to reduce, and ultimately remove, some of these disabling barriers, and that this task is the responsibility of society, rather than the disabled person. The social model of disability focuses on peopleââ¬â¢s attitudes towards disability. These attitudes are many and varied, ranging from prejudice and stereotyping, to unnecessary inflexible organisational practices and procedures.An example of a medical model approach would be a course leader who refuses to produce a hand-out in a larger font for a visually impaired student. The student cannot therefore participate in the class discussion impacting on the students learning development and also could make that student feel isolated and alone. The medical model of disability also affects the way disabled people think about themselves. Many disabled people internalise the negative message that all disabled peopleââ¬â¢s problems stem from not having ââ¬Ënormal' bodies.Disabled people too can be led to believe that their impairments automatically prevent them from participating in social activities. This attitude can make disabled people less likely to challenge their exclusion from mainstream society. Regarding the social model, society is shown to disable people who have impairments becau se the way it has been set up prevents disabled people from taking part in everyday life. It follows that if disabled people are to be able to join in mainstream society, the way society is organised must be changed.Removing the barriers which exclude disabled people who have impairments can bring about thisà change. An example of this would be a course leader meeting with a visually impaired member of the group before the beginning of a course to find out how hand-outs can be adapted so that the student can read them.Positive working practice allows health and social care workers to meet the specific needs of clients. Every area of work needs to make sure that it meets the needs of all individuals with additional needs. Positive working practice becomes a great addition when considering how it can be applied to help those with additional needs. Before this was brought in, it was a common for individuals with additional needs to be expected to fit in with the rest of society meani ng that their needs were not being met. In recent years, this has been changed.Services provide a more patient orientated examination resulting in the patient being directly involved in every decision made. This left all decisive action down to them, ensuring they got the treatment they needed and felt comfortable with.
Friday, January 10, 2020
The Unexpected Truth About Unc Essay Samples
The Unexpected Truth About Unc Essay Samples Unc Essay Samples Secrets The voice you use ought to be approachable in addition to intelligent. By the conclusion of high school, a number of the experiences which you are most proud of don't have a tendency to be the sort of things that wind up on your resume. Apart from repetition of ideas you need to avoid, get away too from using the exact same word in a sentence. For instance, if your preferred topic is the area of literature, you could speak about your experiences with diverse genres or with foreign writers. Life After Unc Essay Samples Now that you know the four key constituents of a great thesis statement, allow me to give you more thesis statement examples. Because personal statements are short, you just won't have enough time to spell out everything you've loved about a specific subject in enough detail to make it count. An excellent thesis statement will accomplish precisely the same thing. Above all, it makes a statemen t. You know the majority of the situations you want to say already. Government surveillance programs do more damage than good since they invade civil liberties, lead innocent individuals to suffer unfair punishments, and ultimately fail to safeguard the citizens they are made to safeguard. One reason that the admissions office wishes to learn which of the applicants has been through something unlike the majority of other people is that they're hoping to raise the number of points of view in the student body. Even more than knowing that you had the ability to fix the issue, colleges wish to observe how you approached the circumstance. Citations and extracts from several sources have to be formatted properly. This document has to be absolutely letter-perfect. Instead, you've got to realize that inside this essay, like in the rest of the essays, the how matters a great deal more than the what. Most significantly, be certain that your essays are quite a reflection of you. Really, the application essays are a lot closer to a brief story. The very first portion of this essay is all about problem-solving. The second section should choose the remainder of the space. This is the location where you show precisely how much you comprehend the worth of what you did, and the way you've changed and grown as a consequence of it. Discover what material you'll be able to cut and cut it. Please describe why you're interested in attending UNC Wilmington. Since first setting foot on campus a couple of years before, I have discovered that Penn has ever stood out in my search for the ideal university. A lot more colleges will commence releasing them over the upcoming several weeks. Please describe why you're interested in attending Appalachian Stat e University. Therefore, if you're not already committed to a particular plan of study, don't worry. For these reasons, programs like PRISM run by the NSA ought to be discontinued. If you anticipate pursuing something similar at UIUC, now's a remarkable chance to express that. Don't be scared to speak to professors or professionals in the area. The Basics of Unc Essay Samples The point is to share the sorts of things which don't wind up on your transcript. But this doesn't need to be the only means you draw this URL. And that's a fairly strong point. Bear in mind that for some things the explanation may be obvious. The cost of an essay rides on the quantity of effort the writer has to exert. Be certain that your essay explains everything the reader needs to know to comprehend what you were facing. Because this essay will necessarily have to devote some time on other folks, you must obtain a great proportion of them-time and me-time. It wants to know whether this mindset of out-of-the-box-ness is something you are already comfortable with.
Thursday, January 2, 2020
Essay on The Wise Ruling and the Unwise Seeking Consent of...
The Wise Ruling and the Unwise Seeking Consent of the Masses The most difficult thing for a regime to achieve is that of acquiring the best ruler, with the consent of the ruled. Aristotle acknowledges this in his works The Politics, and Caius Marcius Coriolanus faces this difficult task in the Shakespeare play The Tragedy of Coriolanus. We even see this same difficult task arise in contemporary politics, as the masses are wooed one way or the other with sound bites, and talk show appearances, by candidates who may not be the best leader for the republic. To this day I dont know if there is a real solution for this dilemma. There is, however, a better way to go about seeking the consent of the ruled then the route Coriolanusâ⬠¦show more contentâ⬠¦He believes that a man without a city is either a beast or a god, since man is not self-sufficient. All partnerships aim at some good. The polis, being a multitude of partnerships, also aims at good. Good, the activity of the soul in conformity with virtue is possible because of the partnerships existing between the citizens of the city. This idea can be a dilemma for any regime, even our own. The key is for two things to occur: 1) the soul must rule the body. 2) Reason must rule over a well-ordered soul. A soul, Aristotle believes is made up of three parts: logos, thymos, and eros. Each part possesses a virtue necessary for justice to rule the soul. This will occur if each virtue minds its own business. Logos is the element of the soul that separates us from the animals. It controls rationality, speech, and reason. An animal can be taught to do many tricks, but an animal cannot be taught to contemplate complex philosophical ideas or quantitative physics equations. Logos must allow wisdom to govern. Thymos is the part of the soul that gives man his will to do the right thing and stand up for what is right. It must be governed by courage. Thymos, in its correct sense is facing danger the way it should be faced. It is brave and virtuous to lead an army onto the battlefield for a noble cause even though the odds may not necessarily be in your favor. It is wrong and stupid to try and take on a whole army byShow MoreRelatedOrganisational Theory230255 Words à |à 922 Pagesexperience and understanding and provided the field with an imaginative resource to address the dynamics between theory and practice. Dr Susanne Tietze, Bradford University, UK The key to success for managers is not only to be result oriented but also to be wise in their decision making. This requires that they have a deeper than superficial understanding of management and organization issues. McAuley et al. helps student and managers understand organizational performance without having to go through extensive
Tuesday, December 24, 2019
Essay on A Study of Gladiator - 1684 Words
A Study of Gladiator This is a study of the 1999 box-office hit, Gladiator, starring Russell Crowe. In this article, I will be looking at camera shots, soundtrack, special effects and other things, that enhance the viewing of the film, and increase its quality. We watched the first forty minutes of the film, and were asked to analyse its media attributes. The film is set in the Caesar period, at the end of the reign of Marco Aurelius. The main opening scene is set in Germania, and it is the beginning of a battle, but the end of a war, between the Roman legion and some barbarians. The very first scene is not a reality shot, its a dream shot. The scene itself is at Maximus home, in hisâ⬠¦show more contentâ⬠¦Having spoken to Quintus, Maximus rides his horse over to the cavalry. I noticed that on the way to the cavalry all of the infantry Maximus passes rises, probably in honour and to show their respect for him. The camera follows Maximus on his horse, while showing all of the soldiers in the background. Then, when Maximus reaches the rest of his cavalry, the camera angle changes, to the view of an onlooker, looking at all of the cavalry surrounding Maximus. Then, the angle again changes to being closer to Maximus, and shows him talking to the men about how if they fall down and wake up in green grass, they are already dead, in the kingdom of Elysium. This is significant because he is giving his men confidence, and he is making them feel better because they may be in the face of death. This also shows the influence Maximus himself has over them, not just because of his authority, but because of his friendship to them. What he says to them is both quite positive, but then also very negative, in saying that they might die, but then saying that if they do they will be in the eternal paradise, Elysium. Having said this, the next shot the camera gives is one of an archer, who is going to give the signal to theShow MoreRelatedThe Gladiator Games Jeffrey Castro1175 Words à |à 5 PagesResearch Paper- The Gladiator Games Jeffrey Castro When you think about the Roman Empire and their culture, it is hard to not think about the gladiator games. To the romans, the gladiator games were more than just a sport. They were a huge part of their history and culture. In our modern times, there are many misconceptions about the gladiator games and the gladiators themselves. The gladiators are often thought of as either slaves being forced to fight to the death or as blood-crazed barbariansRead MoreGladitorial Combat in Ancient Rome Essay766 Words à |à 4 Pagesof the bloodthirsty entertainment was in the form of the gladiator. The word gladiator comes from the Latin for swordsman, from gladius or sword. The first gladiators were part of a sacrificial rite adopted from the Etruscans in 264, BC, nearly 500 years after the founding of Rome (Johnston 238). The sons of Junius Brutus first displayed gladiatorial combat when they honored their father at his funeral by matching three pairs of gladiators. Gladiatorial combat was or iginally part of a religiousRead MoreSummary : Emperor Commodus 1581 Words à |à 7 Pages earlier rebellion, when he mistakenly thought Marcus Aurelius had died, might well have been inspired by a fear of what was to come if Commodus came to the throne. Commodus was a crazed power lunatic who thought he was a God and fought in the Gladiator events thinking he could not die. Commodus was in fact a terrible one. Cruelty, vanity, power and fear formed into a terrifyingly dangerous mix of bloodlust, suspicion and megalomania. 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Rome1179 Words à |à 5 Pagesthe 2004 film Troy, based loosely on Homerââ¬â¢s the Iliad, tells the story of the hero Achilles and the Trojan War. Troy was a critical success, grossing nearly 500 million dollars worldwide. But the movie did not exhibit merely entertainment values: studies found Troy also established on the male demographics the need to possess a strong physique and mentality. It is clearly visible, therefore, that the repre sentations of Greek mythology in modern adaptations can alter our interpretation of the societyââ¬â¢s
Monday, December 16, 2019
Recipe for a Perfect Party Free Essays
So you need to plan a party. Where do you begin? Party planning involves many steps and is essential to being a good host or hostess. Parties can vary from theme, to size, to guests attending. We will write a custom essay sample on Recipe for a Perfect Party or any similar topic only for you Order Now Three major steps in any perfect party is choosing a date, creating a menu, and setting the atmosphere. Choosing a date is the first step in planning a party. For example, if you are planning a Christmas party, chances are your guests will have other parties to attend this time of year and you wonââ¬â¢t want this to interfere with your party.This is also crucial as you need to make your invitations and RSVPââ¬â¢s to know when your guests will or will not be likely to attend. Ask your potential guests when would be the best time for them. This not only gathers information for your party but also relieves the stress in not knowing how much time you have to prepare, including creating a menu. The second step in planning a party is choosing and creating a menu. This can tie into the theme or main reason for your party I. e. : Christmas/New Yearââ¬â¢s Eve, Birthday, Wedding etc.If your theme is Christmas/New Yearââ¬â¢s you may want to go all out and have an expensive menu as opposed to a Birthday party where you may only want to have pot luck. Creating a menu also includes drinks- both hot and cold, desserts and finger foods. Often a key ingredient in parties, drinking allows your guests to socialize and get more acquainted with each other. Make things interesting by having a dessert table served buffet style. You may also want to use this serving style for serving finger foods, or drinks. This way guests can help themselves which may alleviate stress of serving food or beverages at specific times. Such foods/beverages that may be served buffet style may include Do-it-yourself cocktails, fresh fruits and vegetables- suitable for dips, cheese and crackers, or even a chocolate fountain. This is a simple but effective way in making your party a pleasant experience and can also be used in a variety of parties. The second element is whether you will be making the food yourself or hiring a caterer. If you are making the food yourself, take into consideration any dietary requirements of your guests. Are there any guests allergic to anything?Are any guests vegetarian? The last thing you want is someone getting sick or not having anything to eat thus leaving your guest(s) hungry. Also, recognize what time of day your party will be held in relation to the time the food will be served. For instance, if your party is to be held at 2:00pm you are not likely to serve a complete 3 course meal. The third step but not necessarily the final step in planning a perfect party is the creating your atmosphere. This is often the most time consuming step as it involves a variety of things, but can also be the most fun.Creativity is key in setting the perfect atmosphere. Location, music and decorations are covered in this step. Select a location that can be tied into your theme. For example if you are having a winter or Christmas themed party, you may want to host your event at a skating rink. When selecting your location, ask if there are any features- which may be beneficial to your party, such as a sound/light system, dance floor or kitchen area. Most locations that are commonly used for this type of entertaining have some of these features.Music- a key component in parties- can set the mood for a memorable party. Keep things inexpensive by using your own CDs or songs. This saves money on renting a DJ or other music service and you also know what your getting which will help avoid disappointment. Create a song list that relates to your theme. If you are having a New Yearââ¬â¢s Eve party, you may want to include the traditional ââ¬Å"Auld Lang Syneâ⬠or other holiday related songs. You may also want to ask your guests which genre of music or artists they like. Be sure to include both fast and slow music- perfect for dancing! Decorations which are the finishing touch on any party, are a great way in showing off your creativity and ideas. These can vary both big and small, expensive or inexpensive. One easy way to decorate is making your own homemade decorations. For a Christmas party, use a Christmas ornament with your guests name on it as a place card. This doubles as a party favour. Other decorating ideas may include grouping together unscented candles of different heights and colors that suit your theme.This is perfect for some ambiance lighting, and itââ¬â¢s also effective for those who have allergies-because there are no flowers or strong perfumes involved. If your party takes you outside, decorate the outside using simple but attractive white lights. Lights of any color, can be used any time of year and easily adds that festive feel. Among other decorations, offer your guests an area for photo opportunities such as a backdrop that may include a swing to sit on, a festive scene or, if available, something as simple as the scenery. This will allow for others to look back and appreciate your party long after it has ended.As you can see, party planning involves many details, options and time, and can be overwhelming, but it doesnââ¬â¢t have to be. Every party is different and there is no right or wrong way to plan but starting with these three simple steps, you are on your way to planning the perfect party of any theme or type, perfectly. You chose your date, created your menu, and decorated your venue. Also one of (if not) the most important steps in planning a perfect party is to do just that. Party! Have fun, relax and enjoy yourself! P. S- Leave the cleanup for tomorrow, you deserve it! How to cite Recipe for a Perfect Party, Papers
Sunday, December 8, 2019
Relationship of Neanderthals to Modern Humans free essay sample
After extracting ancient DNA from the 40,000-year-old bones of Neanderthals, scientists have obtained a draft sequence of the Neanderthal genome, yielding important new insights into the evolution of modern humans. No other ancient people have aroused more controversy and confusion over the last century and a half than have the Neanderthals (3,4). There is an on-going debate as to whether they should be considered Homo sapiens. While the idea that modern humans originated in Africa and spread out to other parts of the world is widely accepted, several scenarios have been proposed to account for the replacement of neanderthal populations. The multi regional hypothesis holds that neanderthal populations in Eurasia and Africa were held together by gene flow. Fossil and genetic evidence supports an African origin for Modern Humans (1,3,5,9,10). A decade after scientists first cracked the human genome, researchers announced that they have done the same for Neanderthals, the species of hominid that existed from roughly 400,000 to 30,000 years ago, when their closest relatives, early modern humans, may have driven them to extinction (1,3,5,9,10). We will write a custom essay sample on Relationship of Neanderthals to Modern Humans or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Led by ancient-DNA expert Svante Paabo of Germanys Max Planck Institute for Evolutionary Anthropology, scientists reconstructed about 60% of the Neanderthal genome by analyzing tiny chains of ancient DNA extracted from bone fragments of three female Neanderthals excavated in the late 1970s and early 80s from a cave in Croatia (6,8). The bones are 38,000 to 44,000 years old. The genetic information turned up some intriguing findings, indicating, for instance, that at some point after early modern humans migrated out of Africa, they mingled and mated with Neanderthals, possibly in the Middle East or North Africa as much as 80,000 years ago (5,7,10). If that is the case, it occurred significantly earlier than scientists who support the interbreeding hypothesis would have expected. Comparisons with DNA from modern humans show that some Neanderthal DNA has survived to the present (3,4,7). Moreover, by analyzing ancient DNA alongside modern samples, scientists were able to identify a handful of genetic changes that evolved in modern humans sometime after their ancestors and Neanderthals diverged, 440,000 to 270,000 years ago (2,4). Researchers compared the Neanderthal genome with the genomes of five living people: one San from southern Africa, one Yoruba from West Africa, one Papua New Guinean, one Han Chinese and one French person (2,4,6). Scientists discovered that 1% to 4% of the latter three DNA samples is shared with Neanderthals ââ¬â proof that Neanderthals and early modern humans interbred. The absence of Neanderthal DNA in the genomes of the two present-day Africans indicates that interbreeding occurred after some root population of early modern humans left Africa but before the species evolved into distinct groups in Europe and Asia (1,3,5,9,10). All studies of Neanderthal genomic DNA use material obtained from fossilized Neanderthal bones that are tens of thousands of years old. As one might expect, the quality of the material that can be recovered from such specimens is very poor, as DNA degrades over time. Consequently, there is wide variability in DNA preservation among available Neanderthal specimens, and most Neanderthal bones yield no usable DNA (2,3,4). When present, Neanderthal genomic DNA is recovered in short (50- to 100-bp) fragments (2,3,4) The information content of Neanderthal DNA is also degraded: Individual nucleotides are subject to chemical modification, with deamination of cytosine to uracil the most common lesion (2,4). Moreover, the fragments of Neanderthal genomic DNA are mixed with microbial contaminants that constitute gt;90% of the recovered DNA. The amount of DNA damage and microbial contamination are dependent on ambient environmental conditions: The ancient specimens that have provided the most intact DNA are mammoth remains recovered from permafrost. These specimens often include preserved hair shafts and soft tissues from which relatively high-quality DNA can be recovered (2,4,5). None of the Neanderthal specimens providing the DNA for whole-genome sequencing approach this level of preservation (2,4,5,6). In addition to these challenges, ancient specimens frequently become contaminated with modern human DNA during handling and DNA extraction.
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